Preguntas frecuentes

What is the advantage of gellan gum in plant tissue culture?

CAGGEL^TM MTC Gellan Gum for Plant Tissue Culture

Agar is currently the gelling agent of choice for plant tissue culture. However, the presence of impurities or inhibitory factors (including sulfur) in agar can adversely affect growth. CAGGEL^TMMTC offers a promising alternative to agar in this application because of its purity. The similarity between agar and gellan gum has led to the gellan as an alternative to agar for plant tissue culture media. gellan gum can not only replace agar in many routine medium applications, but can also give a higher degree of root growth in certain situations.   

CAGGEL^TM MTC is a kind of special designed low acyl gellan gum which can be used to formulate a suitable growth matrix for many plant tissue culture mediums. .

Ease of Processing with CAGGEL^TM MTC Medium

· CAGGEL^TM MTC gellan gum disperses and hydrates easily in either hot or cold deionized water, forming viscous solutions in cold distilled water.

· In the presence of soluble salts, CAGGEL^TM MTC can be used to provide high gel strength at low CAGGEL^TM MTC concentrations (normally at approximately half the concentration required for agar).

· At high temperatures, the low viscosity of CAGGEL^TM MTC solutions facilitates pipetting, pumping, and pouring upon cooling, CAGGEL^TM MTC solutions gel quickly and uniformly.

· CAGGEL^TM MTC is able to withstand normal autoclaving conditions.

· CAGGEL^TMMTC is generally resistant to enzymatic degradation.

· CAGGEL^TMMTC itself is chemically inert to most plant growth media additives

Quality of Plant Tissue Culture Medium Prepared with CAGGEL^TM MTC

· CAGGEL^TM MTC gellan gum gels have proven to be a suitable growth matrix for a wide variety of plants, including those traditionally cultured on agar plates as well as other species not easily grown on other substances.

· CAGGEL^TM MTC gels are exceptionally clear, making them an excellent analytical tool.

· CAGGEL^TM MTC gels have essentially the same shelf life as agar gels.

Table 1. Recommend dosage for part of plant tissue culture mediums

Advantages of CAGGEL^TM MTC Compared to Agar

l CAGGEL^TM MTC gellan gum may be used at approximately half the use level of agar

l CAGGEL^TM MTC, produced by a tightly-controlled fermentation process, has consistent product quality.  CAGGEL^TM MTC is unaffected by the vagaries of natural conditions which affect the basic properties of agar.

l CAGGEL^TM MTC gels are remarkably clear in comparison to those formed with agar.

l Gels prepared with CAGGEL^TM MTC set faster than those made with agar. In plant tissue culture applications this reduces preparation time.

l Gels prepared with CAGGEL^TM MTC are stable at high temperatures. In medium sterilization process, the gel remains high gel strength.

l CAGGEL^TM MTC contains no contaminating matters (e.g., phenolic compounds) as those found in agar that are toxic to certain sensitive organisms.