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Is bacteriological agar the same as pure agarose?

Is bacteriological agar the same as pure agarose?

No, bacteriological agar and agarose are distinct commercial products with different chemical purity standards. Bacteriological agar is a highly refined but complete agar extract containing both agarose (the gelling fraction) and a controlled, low percentage of agaropectin (the charged fraction), with a sulfate content typically under 1.0% and ash under 3.0%. Agarose is an premium derivative isolated by completely removing the agaropectin fraction through solvent fractionation or physical precipitation. Pure agarose exhibits near-zero sulfate content ( 0.15%), minimal ash (0.5%), and specialized electroendosmosis (EEO) ratings, making it exclusive to molecular biology applications like DNA/RNA gel electrophoresis.


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