info@cagcolloids.com    +86-198 8490 8291
Menu
Products and Ingredients
FAQs
Nature Refined, Quality Defined
Green Science for better living
Home/

FAQs

How are the phosphate precipitation and alkali precipitation tests conducted for bacteriological-grade agar?

How are the phosphate precipitation and alkali precipitation tests conducted for bacteriological-grade agar?

  • Alkali Precipitation Test:

    Dissolve 1.5 g of agar powder in 100 ml of purified water, adjust the pH to 8.4 ± 0.1 using NaOH. After sterilization in an autoclave at 121°C for 15 minutes, inspect the uncongealed solution in a darkroom by shining a flashlight vertically or parallel to the conical flask. The sample is considered qualified if no precipitation is observed.

  • Phosphate Precipitation Test:

    Dissolve 1.5 g of agar powder in 100 ml of purified water, then add 0.5% K₂HPO₄. After sterilization in an autoclave at 121°C for 15 minutes, inspect the uncongealed solution in a darkroom by shining a flashlight vertically or parallel to the conical flask. The sample is considered qualified if no precipitation is observed


  • Need support on product development?